Rabbit Anti-CALR Polyclonal Antibody, Unconjugated

  • Rabbit Anti-CALR Polyclonal Antibody, Unconjugated

    from ProSci
    CatalogueID : 30-005

  • Contact Vendor

Target CALR
Species Cross Reactivity Homo sapiens, Mus musculus, Rattus norvegicus, Canis lupus familiaris
Host Species Oryctolagus cuniculus
Target Tag/Conjugate Unconjugated
Applications WB
Target/Molecule Synonym CALR, calreticulin, RO, SSA, cC1qR
Unit 0.05 mg
Format Purification: Rabbit Ig G is purified by peptide affinity chromatography method.
Description Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium.Accession Number: NP_004334MW: 48kDaImmunogen: Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CALR.Application: CALR antibody can be used for Western blot at a suggested dilution at 1.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.Positive Control 1: Cat. No. 1211 - HepG2 Cell LysateBuffer: Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100µl of distilled water making the final concentration 1 mg/ml.Storage: Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Cite This Product ProSci cat# 30-005 RRID:AB_1945436
Company ProSci, Inc
Type Polyclonal Antibody
Uniprot ID P15253