Rabbit Anti-Human Calreticulin Polyclonal Antibody, Unconjugated

  • Rabbit Anti-Human Calreticulin Polyclonal Antibody, Unconjugated

    from ProSci
    CatalogueID : 32-125

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Target Calreticulin
Species Cross Reactivity Homo sapiens
Host Species Oryctolagus cuniculus
Target Tag/Conjugate Unconjugated
Applications EIA, WB
Target Species Homo sapiens
Target/Molecule Synonym RO, CRT, SSA, cC1qR, FLJ26680, CALR
Unit 0.1 ml
Format Formulation: Rabbit anti-serum. Purified antibody available at no extra cost.
Description Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.GI Number: 811Immunogen: Synthetic peptide corresponding to aa (E-E-E-D-V-P-G-Q-A-K-D-E-L-C)of human CalreticulinApplication: Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.Positive Control 1: Cat. No. 1201 - HeLa Cell LysateStorage: Calreticulin antibody can be stored at -20ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product ProSci cat# 32-125 RRID:AB_1945437
Company ProSci, Inc
Type Polyclonal Antibody
Uniprot ID P15253