Mouse Anti-Human GLI1 Polyclonal Antibody, Unconjugated

  • Mouse Anti-Human GLI1 Polyclonal Antibody, Unconjugated

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    CatalogueID : 49-519

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Target gli1
Species Cross Reactivity Homo sapiens
Host Species Mus musculus
Target Tag/Conjugate Unconjugated
Applications EIA, WB, IHC
Target Species Homo sapiens
Target/Molecule Synonym GLI1, GLI, gli-1, glioma-associated oncogene, glioma-associated oncogene homolog
Unit 0.05 mg
Format Purification: Delipidation and DefibrinationFormulation: Volume: 50 µl
Description This antiserum was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places Gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that Gli is expressed in embryonal carcinoma cells but not in most adult tissue. Gli has been localized to 12q13-q14.3 by Southern blot analysis. In mice, the gene is located on chromosome 10. In mice, three zinc finger transcription factors, Gli-1, Gli-2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli-1 expression is central and probably sufficient for tumor development in humans. This antibody has been tested in Western blotting with cells expressing human Gli-1 and in IHC-P with human tissue samples.Immunogen: GLI1 antibody was raised against amino acids 805-820 of GLI1. (Mouse)Application: GLI1 antibody can be used for detection of GLI1 by WB (1:2000 - 1:10000), ELISA (1:20000 - 1:100000), IHC-parafin (1:100).Buffer: GLI1 antibody is supplied in 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.Storage: GLI1 antibody can be stored at 4ºC or -20ºC, Avoid repeated freezing and thawing.
Cite This Product ProSci cat# 49-519 RRID:AB_1946512
Company ProSci, Inc
Type Polyclonal Antibody
Uniprot ID P47806