Mouse Anti-GST Monoclonal Antibody, Unconjugated, Clone 6G9B9

  • Mouse Anti-GST Monoclonal Antibody, Unconjugated, Clone 6G9B9

    from ProSci
    CatalogueID : 32-166

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Target GST
Host Species Mus musculus
Target Tag/Conjugate Unconjugated
Applications EIA, WB
Clone Clone 6G9B9
Unit 0.1 mg
Format Purification: Antibody is purified by protein A affinity chromatography.Formulation: Purified antibody in PBS containing 0.03% sodium azide
Description Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.Immunogen: Ni-NTA purified recombinant GST expressed in E. Coli strain M15.Application: Western Blot:Dilution 1:2000 - 5,000 ELISA:Proposed dilution 1:10,000. Determining optimal working dilutions by titration test.Storage: GST monoclonal antibody can be stored at -20ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product ProSci cat# 32-166 RRID:AB_1946691
Company ProSci, Inc
Type Monoclonal Antibody
Isotype IgG1