Mouse Anti-GFP Monoclonal Antibody, Unconjugated, Clone 4B10B2

  • Mouse Anti-GFP Monoclonal Antibody, Unconjugated, Clone 4B10B2

    from ProSci
    CatalogueID : 32-160

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Target GFP
Host Species Mus musculus
Target Tag/Conjugate Unconjugated
Applications EIA, WB
Clone Clone 4B10B2
Unit 0.1 ml
Format Formulation: Purified antibody in PBS containing 0.03% sodium azide
Description GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.Immunogen: Ni-NTA purified full-length recombinant GFP-His expressed in E. Coli strain BL21 (DE3).Application: Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.Storage: GFP monoclonal antibody can be stored at -20ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product ProSci cat# 32-160 RRID:AB_1946486
Company ProSci, Inc
Type Monoclonal Antibody
Isotype IgG2a