Rabbit Anti-GFP Polyclonal Antibody, Unconjugated

  • Rabbit Anti-GFP Polyclonal Antibody, Unconjugated

    from ProSci
    CatalogueID : 24020

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Target GFP
Species Cross Reactivity Homo sapiens, Mus musculus, Rattus norvegicus
Host Species Oryctolagus cuniculus
Target Tag/Conjugate Unconjugated
Applications EIA, WB, IHC
Unit 0.1 mg
Format Purification: Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.Formulation: PRESERVATIVE: 0.01% (w/v) Sodium Azide
Description Affinity Purified anti-Green Fluorescent Protein (Aequorea victoria) [Rabbit] Minimum Cross Reactivity to Human, Mouse and Rat Serum ProteinsImmunogen: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.Preparation: PHYSICAL STATE: Liquid (sterile filtered) ANTIBODY CONCENTRATION: 1.0 mg/ml (by UV absorbance at 280 nm)Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2Applications: Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Recommended dilutions are as follows, ELISA 1:15,000 - 1:60,000, Western Blot 1:500 - 1:5,000, Immunohistochemistry 1:200 - 1:3,000. (Optimal dilutions should be determined by user).Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. Forextended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
Cite This Product ProSci cat# 24020 RRID:AB_1941118
Company ProSci, Inc
Type Polyclonal Antibody