Mouse Anti-GAPDH Monoclonal Antibody, Unconjugated

  • Mouse Anti-GAPDH Monoclonal Antibody, Unconjugated

    from ProSci
    CatalogueID : 50-267

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Target GAPDH
Species Cross Reactivity Homo sapiens, Mus musculus, Rattus norvegicus, Bos taurus, Gallus gallus, Primate (Species unspecified)
Host Species Mus musculus
Target Tag/Conjugate Unconjugated
Applications WB
Unit 0.1 ml
Format Purification: Unpurified, concentrated culture supernatant
Description Biological Significance: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.Specificity: Specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.Immunogen: Extensively purified pig GAPDH.Buffer: GAPDH antibody is supplied as total IgG fraction.Storage: GAPDH antibody can be stored at -20ºC and is stable at -20ºC for at least 1 year.
Cite This Product ProSci cat# 50-267 RRID:AB_1946446
Company ProSci, Inc
Type Monoclonal Antibody
Isotype IgM
Uniprot ID P16858