Mouse Anti-Human GAPDH Monoclonal Antibody, Unconjugated, Clone 2D4A7

  • Mouse Anti-Human GAPDH Monoclonal Antibody, Unconjugated, Clone 2D4A7

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    CatalogueID : 32-159

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Target GAPDH
Species Cross Reactivity Homo sapiens
Host Species Mus musculus
Target Tag/Conjugate Unconjugated
Applications EIA, WB, IHC, IF
Clone Clone 2D4A7
Target Species Homo sapiens
Target/Molecule Synonym G3PD, GAPD, MGC88685
Unit 0.1 ml
Format Formulation: Ascitic fluid containing 0.03% sodium azide. Purified antibody available at no extra cost.
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer’s and Huntington’s disease.GI Number: 2597Immunogen: Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3).Application: Western Blot:1:500 - 1:2,000 IHC(P/F):1:200 - 1:1,000 IF:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.Positive Control 1: Cat. No. 1201 - HeLa Cell LysatePositive Control 2: Cat. No. 1205 - Jurkat Cell LysateStorage: GAPDH monoclonal antibody can be stored at -20ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product ProSci cat# 32-159 RRID:AB_1946444
Company ProSci, Inc
Type Monoclonal Antibody
Isotype IgG1
Uniprot ID P16858