Rabbit Anti-GCLC Polyclonal Antibody, Unconjugated

  • Rabbit Anti-GCLC Polyclonal Antibody, Unconjugated

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    CatalogueID : 49-852

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Target Gclc
Species Cross Reactivity Homo sapiens, Mus musculus, Bos taurus, Monkey (Species unspecified)
Host Species Oryctolagus cuniculus
Target Tag/Conjugate Unconjugated
Applications EIA, WB, IHC, IP
Target/Molecule Synonym GCLC, EC 6.3.2.2, GAMMA-ECS, GCS, GLCL, GLCLC
Unit 0.05 mg
Format Purification: Protein A ColumnFormulation: Volume: 50 µl
Description Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH. Western Blot (Ab 2.5-5ug/ml for 2hrs at RT) Immunoprecipitation (Denatured verified) (Use Protein A) (Ab 10ug/mg protein lysate) Positive Control Jurkat, MAD109, PC12 cells Cellular Localization Cytoplasmic Recognizes the heavy subunit of GCS. Species Crossreactivity: Human, Monkey, Mouse, and Rat, Bovine (weak).Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene for catalytic subunit (GLCLC) encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p21-p22. GLCLC deficiency in human is associated with enzymopathic hemolytic anemia.Immunogen: GCLC antibody was raised against a synthetic peptide derived from corresponding to aa295-313 of GCLC. (Rat)Application: GCLC antibody can be used for detection of GCLC by WB, IP, ELISA, IHC-parafin (minimum working concentration: 5 µg/ml maximum working concentration: 5 µg/ml).Buffer: GCLC antibody is supplied in 10 mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, 40% glycerol.Storage: GCLC antibody can be stored at 4ºC for short term storage, and -20ºC with 40-50% glycerol for long term storage.
Cite This Product ProSci cat# 49-852 RRID:AB_1946463
Company ProSci, Inc
Type Polyclonal Antibody