Rabbit Anti-HIF3A Polyclonal Antibody, Unconjugated

  • Rabbit Anti-HIF3A Polyclonal Antibody, Unconjugated

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    CatalogueID : 49-586

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Target Hif3a
Species Cross Reactivity Homo sapiens, Rattus norvegicus
Host Species Oryctolagus cuniculus
Target Tag/Conjugate Unconjugated
Applications EIA, WB, IHC, IF
Target/Molecule Synonym HIF3A, HIF-3A, HIF-3A4, IPAS, MOP7, PASD7
Unit 0.05 mg
Format Purification: Immunoaffinity ChromatographyFormulation: Weight: 50 µg Concentration: 1.2 mg/ml
Description One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits (HIF1-a, HIF2-a, or HIF3-a) bound to the aryl hydrocarbon receptor nuclear translocator (ARNT) that is also known as HIF1-b. HIF-a is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and angiogenesis . Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxia-inducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as CREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.Immunogen: HIF3A antibody was raised against aa 581-592 of HIF3A. (Mouse)Application: HIF3A antibody can be used for detection of HIF3A by WB (1:100 - 1:8000), ELISA (1:14000 - 1:80000), IF, IHC-parafin (5 µg/ml).Buffer: HIF3A antibody is supplied in 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.Storage: HIF3A antibody can be stored at -20ºC prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20ºC or below. Avoid cycles of freezing and thawing.
Cite This Product ProSci cat# 49-586 RRID:AB_1946828
Company ProSci, Inc
Type Polyclonal Antibody