Streptavidin-NC

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Format Purity: Greater than 90% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Format: 0.5 mg/ml glycerol.
Target Tag/Conjugate Unconjugated
Target/Molecule Synonym Streptavidin-NC
Source/Expression System E. coli
Original Item Name Streptavidin-NC
Molecule Name Streptavidin-NC
Category Protein
Unit 0.02 mg
Description A 60kD extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN. streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.Recombinant Streptavidin-NC produced in E.Coli is a single. non-glycosylated. polypeptide chain having a molecular mass of 24kDa. Recombinant Streptavidin-NC not only binds to nitrocellulose membrane readily but also preserves the full biotin binding ability. Recombinant Strptavidin is purified by proprietary chromatographic techniques. Testing Protocols: Calibrators and controls for immunoassays and western blot standards. Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Physical Appearance: Sterile filtered liquid formulation. Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Recombinant Strptavidin. Product ID: 45623