Human Ubiquitin Conjugating enzyme 9

  • Human Ubiquitin Conjugating enzyme 9

    CatalogueID : GWB-17D99E

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Species Human
Target/Molecule Synonym Ubiquitin conjugating enzyme 9, SUMO-1-conjugating enzyme UBC9, hUbc9 (E2), EC 6.3.2.19, SUMO-1-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBE2I, UBC9, UBCE9
Target Tag/Conjugate Unconjugated
Format Purity: >= 95% by SDS PAGE
Format: Liquid. In 50mM HEPES (pH7.5) 150mM NaCl. 1mM DTT. 10% Glycerol.
Applications SDS
Original Item Name Ubiquitin Conjugating enzyme 9 human
Source/Expression System E. coli
Activity Catalytic Activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Category Protein
References [1] Wang. Z. Y. Qiu. Q. Q. Seufert. W. Taguchi. T. Testa. J. R. Whitmore. S. A. Callen. D. F. Welsh. D. Shenk. T. and Deuel. T. F.
Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9
[2] Wang. Z. Y. and Deuel. T. F. et al.
Direct Submission
[3] Yasugi T. Howley P. M.
Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9.
[4] Tachibana M. Iwata N. Watanabe A. Nobukuni Y. Ploplis B. Kajigaya S.
Assignment of the gene for a ubiquitin-conjugating enzyme (UBE2I) to human chromosome band 16p13. 3 by in situ hybridization.
[5] Watanabe T. K. Fujiwara T. Kawai A. Shimizu F. Takami S. Hirano H. Okuno S. Ozaki K. Takeda S. Shimada Y. et al.
Cloning. expression. and mapping of UBE2I. a novel gene encoding a human homologue of yeast ubiquitin-conjugating enzymes which are critical for regulating the cell cycle.
[6] Masson M. Menissier-de Murcia J. Mattei M. -G. de Murcia G. M. Niedergang C. P.
Poly(ADP-ribose) polymerase interacts with a novel human ubiquitin conjugating enzyme: hUbc9.
[7] Kovalenko O. V. Plug A. W. Haaf T. Gonda D. K. Ashley T. Ward D. C. Radding C. M. Golub E. I.
Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes.
[8] Jiang W. Koltin Y.
Two-hybrid interaction of a human UBC9 homolog with centromere proteins of Saccharomyces cerevisiae.
[9] Wang Z. -Y. Qiu Q. Seufert W. Taguchi T. Testa J. R. Whitmore S. A. Callen D. F. Welsh D. Shenk T. Deuel T. F. et al.
Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.
[10] Shen Z.
Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
EC Number EC 6.3.2.19
Accession Number Accession Number: AAC50716.1
Sp Accession Number: P63279
Molecule Name Ubiquitin Conjugating enzyme 9
Unit 0.5 mg
Description Human Ubc9 is homologous to ubiquitin-conjugating enzymes (E2s). However. instead of conjugating ubiquitin. it conjugates a ubiquitin homologue. small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and –conjugating (E2) enzymes.Function: Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins.Catalytic Activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.Pathway: SUMO conjugation; second step.Subunit: Forms a tight complex in association with two RAN GTPase interacting proteins. RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2 (By similarity). Interacts with SIAH1 and PARP.Tissue Specificity: Expressed in heart. skeletal muscle. pancreas. kidney. liver. lung. placenta and brain. Also expressed in testis and thymus.Similarity: Belongs to the ubiquitin-conjugating enzyme family. Sequence Length (aa): 158 MW: 18007 OMIM: 601661; gene. [ NCBI / EBI] Applications Tested: SDS Applications Not Tested: MS Testing Protocols: SDS: 14% SDS PAGE||10-002-38076_SD Cross-Reactivity: Human Storage: Store at -20°C. Avoid frequent freeze and thaw. Stability: 6-12 months at -70°C. Sequence: MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS Product ID: 38076