Proteasome subunit beta type 4

  • Proteasome subunit beta type 4

    CatalogueID : GWB-549098

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Format Purity: 0.95
Format: 10 mM Tris. pH 8.0. 0.1% Triton X-100. 0.002% NaN3. 10mM DTT.
Target Tag/Conjugate Unconjugated
Target/Molecule Synonym Proteasome (prosome. macropain) subunit. beta type. 4, Proteasome subunit. beta type. 4, EC 3.4.25.1, Proteasome beta chain, Macropain beta chain, Multicatalytic endopeptidase complex beta chain, Proteasome chain 3, HSN3, HsBPROS26, PSMB4, HN3, HsN3, PROS26
Category Protein
Applications MS, SDS
Accession Number Accession Number: NP_002787.1
Sp Accession Number: P28070
Source/Expression System E. coli
References [1] McCusker D. Jones T. Sheer D and Trowsdale J. et al.
Genetic relationships of the genes encoding the human proteasome beta subunits and the proteasome PA28 complex
[2] Nothwang. H. G. Tamura. T. Tanaka. K. and Ichihara. A. et al.
Sequence analyses and inter-species comparisons of three novel human proteasomal subunits. HsN3. HsC7-I and HsC10-II. confine potential proteolytic active-site residues
[3] Gerards WL. Hop FW. Hendriks IL and Bloemendal H. et al.
Cloning and expression of a human pro(tea)some beta-subunit cDNA: a homologue of the yeast PRE4-subunit essential for peptidylglutamyl-peptide hydrolase activity
[4] . Nothwang H. G. Tamura T. Tanaka K. Ichihara A.
Sequence analyses and inter-species comparisons of three novel human proteasomal subunits. HsN3. HsC7-I and HsC10-II. confine potential proteolytic active-site residues.
[5] . Kalnine N. Chen X. Rolfs A. Halleck A. Hines L. Eisenstein S. Koundinya M. Raphael J. Moreira D. Kelley T. et al.
Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
[6] . Strausberg R. L. Feingold E. A. Grouse L. H. Derge J. G. Klausner R. D. Collins F. S. Wagner L. Shenmen C. M. Schuler G. D. Altschul S. F. et al.
Generation and initial analysis of more than 15. 000 full-length human and mouse cDNA sequences.
[7] . Gerards W. L. Hop F. W. Hendriks I. L. Bloemendal H.
Cloning and expression of a human pro(tea)some beta-subunit cDNA: a homologue of the yeast PRE4-subunit essential for peptidylglutamyl-peptide hydrolase activity.
[8] . Lee L. W. Moomaw C. R. Orth K. McGuire M. J. DeMartino G. N. Slaughter C. A.
Relationships among the subunits of the high molecular weight proteinase. macropain (proteasome).
[9] . Hochstrasser D. F. Frutiger S. Paquet N. Bairoch A. Ravier F. Pasquali C. Sanchez J. -C. Tissot J. -D. Bjellqvist B. Vargas R. et al.
Submitted (JUN-1992) to Swiss-Prot.
[10] . Kristensen P. Johnsen A. H. Uerkvitz W. Tanaka K. Hendil K. B.
Human proteasome subunits from 2-dimensional gels identified by partial sequencing.
EC Number EC 3.4.25.1
Original Item Name Proteasome subunit beta type 4
Molecule Name Proteasome subunit beta type 4
Activity Catalytic Activity: Cleavage of peptide bonds with very broad specificity.
Unit 0.05 mg
Description Function: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg. Phe. Tyr. Leu. and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.Catalytic Activity: Cleavage of peptide bonds with very broad specificity.Pathway: Involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway.Subunit: The proteasome is composed of at least 15 non identical subunits which form a highly ordered ring-shaped structure.Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity).Similarity: Belongs to the peptidase T1B family [view classification]. Chromosome Location: 1q21 Sequence Position 1: 1 Sequence Position 2: 264 Sequence Length (aa): 264 MW: 29192 KEGG pathway: Proteasome 03050Reactome Event:Cell Cycle (Mitotic) 69278Reactome Event:Cell Cycle Checkpoints 69620Reactome Event:DNA Replication 69306 OMIM: 602177; gene. [ NCBI / EBI] Applications Tested: MS. SDS Applications Not Tested: ELISA. Western Blot Testing Protocols: SDS: Analysis of PSMB4 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.||10-288-22554F_SD Cross-Reactivity: human Storage: -70°C. Avoid frequent freeze and thaw. Stability: 6-12 months at -70°C. Fusion: His-tag at N-terminus. Fusion: His-tag at N-terminus. Product ID: 5300