Human Protein Disulfide Isomerase (PDI) , N-terminal 6xHis Conjugat...

  • Human Protein Disulfide Isomerase (PDI) , N-terminal 6xHis Conjugated/Tagged

    CatalogueID : GWB-2D13C5

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Format Purity: Greater than 95% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Format: The protein (1 mg/ml) was lyophilized with 50 mM potassium phosphate buffer pH=7.5.
Species Human
Target Tag/Conjugate N-terminal 6xHis
Target/Molecule Synonym PDI
Molecule Name Protein Disulfide Isomerase (PDI)
Category Protein
Original Item Name Protein Disulfide Isomerase (PDI) Human
Source/Expression System E. coli
Unit 0.1 mg
Description Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation. reduction. or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). They act also as chaperones. and are. therefore. part of a quality-control system for the correct folding of the proteins in the same subcellular compartment. PDI has been foundto have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.Recombinant Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization. as well as the reduction of disulphide bonds in proteins. Recombinant PDI has been foundto have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. Recombinant Human PDI produced in E.Coli is a single. non-glycosylated. polypeptide chain containing 508 amino acids and having a molecular mass of 57116 Dalton. Human PDI is containsN-terminal 6xHis-tag and purified by proprietary chromatographic techniques. Cross-Reactivity: Human Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.Solubility: It is recommended to reconstitute the lyophilized Recombinant PDI in sterile 18MolarOmega-cm H2O not less than 100ug/ml. which can then be further diluted to other aqueous solutions.Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.Solubility: It is recommended to reconstitute the lyophilized Recombinant PDI in sterile 18MolarOmega-cm H2O not less than 100ug/ml. which can then be further diluted to other aqueous solutions.Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Physical Appearance: Sterile Filtered White Lyophilized (freeze-dried) Powder. Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Recombinant Human PDI. Product ID: 45290