Serine / threonine-protein kinase PAK 1

  • Serine / threonine-protein kinase PAK 1

    CatalogueID : 10-782-55078

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Target/Molecule Synonym P21-activated kinase 1, EC 2.7.11.1, p21-activated kinase 1, PAK-1, P65-PAK, Alpha-PAK, PAK1
Target Tag/Conjugate Unconjugated
Activity Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.
Molecule Name Serine / threonine-protein kinase PAK 1
Source/Expression System Insect Cells
References [1] Eppinga. R. D. Li. Y. Lin. J. L. Mak. A. S. and Lin. J. J. et al.
Requirement of reversible caldesmon phosphorylation at P21-activated kinase-responsive sites for lamellipodia extensions during cell migration
[2] Pavey. S. Zuidervaart. W. van Nieuwpoort. F. Packer. L. Jager. M. Gruis. N. and Hayward. N.
Increased p21-activated kinase-1 expression is associated with invasive potential in uveal melanoma
[3] Even-Faitelson. L. and Ravid. S. et al.
PAK1 and aPKCzeta regulate myosin II-B phosphorylation: a novel signaling pathway regulating filament assembly
[4] Schmidt. M. H. Husnjak. K. Szymkiewicz. I. Haglund. K. and Dikic. I. et al.
Cbl escapes Cdc42-mediated inhibition by downregulation of the adaptor molecule betaPix
[5] Holm. C. Rayala. S. Jirstrom. K. Stal. O. Kumar. R. and Landberg. G. et al.
Association between Pak1 expression and subcellular localization and tamoxifen resistance in breast cancer patients
[6] Sells M. A. Knaus U. G. Bagrodia S. Ambrose D. M. Bokoch G. M. Chernoff J.
Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells.
[7] Brown J. L. Stowers L. Baer M. Trejo J. Coughlin S. Chant J.
Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.
[8] Reid T. Aspenstrom P. Bertoglio J.
Human PAK1B.
[9] Manser E. Huang H. Y. Loo T. H. Chen X. Q. Dong J. M. Leung T. Lim L.
Expression of constitutively active alpha-PAK reveals effects of the kinase on actin and focal complexes.
[10] Frost J. A. Khokhlatchev A. Stippec S. White M. A. Cobb M. H.
Differential effects of PAK1-activating mutations reveal activity-dependent and -independent effects on cytoskeletal regulation.
Accession Number Accession Number: NP_002567.3
Sp Accession Number: Q13153
EC Number EC 2.7.11.1
Original Item Name Serine/threonine-protein kinase PAK 1
Category Protein
Unit 0.05 mg
Description Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Function: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Activity is inhibited in cells undergoing apoptosis. potentially due to binding of CDC2L1 and CDC2L2.Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.Cofactor: Magnesium.Enzyme Regulation: Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer. enables phosphorylation of Thr-423 and allows the kinase domain to adopt an active structure. Also activated by binding to GTP-bound CDC42. independent of the phosphorylation state of Thr-423. Phosphorylation of Thr-84 by OXSR1 inhibits this activation (By similarity).Subunit: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2. p110C. but not the full-length proteins. Component of cytoplasmic complexes. which also contain PXN. ARHGEF6 and GIT1.Subcellular Location: Cytoplasm. Cell membrane; cell-matrix junction; focal adhesion. Recruited to focal adhesions upon activation.Ptm: Autophosphorylated when activated by CDC42/p21 and RAC1.Similarity: Belongs to the Ser/Thr protein kinase family. STE20 subfamily.Similarity: Contains 1 CRIB domain.Similarity: Contains 1 protein kinase domain.Summary: PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins. a family of serine/threonine p21-activating kinases. include PAK1. PAK2. PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found. but their full-length natures have not yet been determined. Sequence Length (aa): 545 MW: 60647 KEGG pathway: Axon guidance 04360KEGG pathway: Epithelial cell signaling in Helicobacter pylori infection 05120KEGG pathway: Focal adhesion 04510KEGG pathway: MAPK signaling pathway 04010KEGG pathway: Natural killer cell mediated cytotoxicity 04650KEGG pathway: Regulation of actin cytoskeleton 04810KEGG pathway: T cell receptor signaling pathway 04660 OMIM: 602590; gene. [ NCBI / EBI] Testing Protocols: Purity: ||10-782-55078 Storage: Store product at -70°C. For optimal storage. aliquot target into smaller quantities after centrifugation and store at recommended temperature. Stability: 1 year at -20°C. Product ID: 55078