G-P-R

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Format Purity: > 95%
Target/Molecule Synonym Gpr [Bacillus licheniformis DSM 13], Germination protease, EC 3.4.24.78, Spore protease, GPR endopeptidase, Germination proteinase
Target Tag/Conjugate Unconjugated
Original Item Name G-P-R
EC Number EC 3.4.24.78
Molecule Name G-P-R
Category Peptide
Accession Number NCBI Accession Number: AAU41617.1
Swiss Protein Accession Number: Q65H47
References [1] Conlon.J.M.. Nielsen.P.F. and Youson.J.H.. et al.
Primary structures of glucagon and glucagon-like peptide isolated from the intestine of the parasitic phase lamprey Petromyzon marinus
Unit 5 mg
Description The GPR peptide sequence (28 amino acids) encompassed the consensus sequence defined by the four GPR motifs conserved in the family of AGS3 proteins. The GPR consensus peptide effectively prevented the binding of AGS3 to Gialpha1.2 in protein interaction assays. inhibited guanosine 5'-O-(3-thiotriphosphate) binding to Gialpha. and stabilized the GDP-bound conformation of Gialpha. The GPR peptide had little effect on nucleotide binding to Goalpha and brain G-protein indicating selective regulation of Gialpha. Thus. the GPR peptide functions as a guanine nucleotide dissociation inhibitor for Gialpha. The GPR consensus peptide also blocked receptor coupling to Gialphabetagamma indicating that although the AGS3-GPR peptide stabilized the GDP-bound conformation of Gialpha. this conformation of Gialpha(GDP) was not recognized by a G-protein coupled receptor.Function: Initiates the rapid degradation of small. acid-soluble proteins during spore germination (By similarity).Catalytic Activity: Endopeptidase action with P4 Glu or Asp. P1 preferably Glu > Asp. P1' hydrophobic and P2' Ala.Subunit: Homotetramer (By similarity).Ptm: Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41. which is active only during spore germination (By similarity).Similarity: Belongs to the peptidase M63 family [view classification]. Entry Name: GPR_BACLD Length (aa): 368 MW: 40443 Storage: 4°C for short term (weeks) and -20°C for long term. Stability: 6-12 months at -20°C. Sequence (One Letter Code): GPRSequence: {GLY} {PRO} {ARG} Formula: C13H24N6O4