Serine / threonine-protein phosphatase PP1-gamma catalytic subunit

  • Serine / threonine-protein phosphatase PP1-gamma catalytic subunit

    CatalogueID : 10-288-22549F

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Format Purity: 0.95
Format: 10 mM Tris. pH 8.0. 0.1% Triton X-100. 0.002% NaN3. 10mM DTT.
Target Tag/Conjugate Unconjugated
Target/Molecule Synonym Protein phosphatase 1. catalytic subunit. gamma isoform, EC 3.1.3.16, PP-1G, Protein phosphatase 1C catalytic subunit, PPP1CC, PPP1G
Applications MS, SDS
Accession Number Accession Number: NP_002701.1
Sp Accession Number: P36873
Original Item Name Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
Category Protein
Source/Expression System E. coli
EC Number EC 3.1.3.16
References [1] Ammosova. T. Jerebtsova. M. Beullens. M. Lesage. B. Jackson. A. Kashanchi. F. Southerland. W. Gordeuk. V. R. Bollen. M. and Nekhai. S.
Nuclear targeting of protein phosphatase-1 by HIV-1 Tat protein
[2] Jones. J. A. Rawles. R. and Hannun. Y. A. et al.
Identification of a novel phosphatidic acid binding domain in protein phosphatase-1
[3] Ammosova. T. Jerebtsova. M. Beullens. M. Voloshin. Y. Ray. P. E. Kumar. A. Bollen. M. and Nekhai. S.
Nuclear protein phosphatase-1 regulates HIV-1 transcription
[4] Eto. M. Elliott. E. Prickett. T. D. and Brautigan. D. L. et al.
Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation
[5] Jones. J. A. and Hannun. Y. A. et al.
Tight binding inhibition of protein phosphatase-1 by phosphatidic acid. Specificity of inhibition by the phospholipid
[6] . Barker H. M. Craig S. P. Spurr N. K. Cohen P. T. W.
Sequence of human protein serine/threonine phosphatase 1 gamma and localization of the gene (PPP1CC) encoding it to chromosome bands 12q24. 1-q24. 2.
[7] . Strausberg R. L. Feingold E. A. Grouse L. H. Derge J. G. Klausner R. D. Collins F. S. Wagner L. Shenmen C. M. Schuler G. D. Altschul S. F. et al.
Generation and initial analysis of more than 15. 000 full-length human and mouse cDNA sequences.
[8] . Norman S. A. Mott D. M.
Molecular cloning and chromosomal localization of a human skeletal muscle PP-1 gamma 1 cDNA.
[9] . MacKintosh R. W. Dalby K. N. Campbell D. G. Cohen P. T. Cohen P. T. W. MacKintosh C.
The cyanobacterial toxin microcystin binds covalently to cysteine-273 on protein phosphatase 1.
[10] . Armstrong C. G. Browne G. J. Cohen P. Cohen P. T. W.
PPP1R6. a novel member of the family of glycogen-targeting subunits of protein phosphatase 1.
Molecule Name Serine / threonine-protein phosphatase PP1-gamma catalytic subunit
Activity Catalytic Activity: A phosphoprotein + H2O = a protein + phosphate.
Unit 0.1 mg
Description Function: Protein phosphatase 1 (PP1) is essential for cell division. and participates in the regulation of glycogen metabolism. muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.Catalytic Activity: A phosphoprotein + H2O = a protein + phosphate.Cofactor: Binds 1 iron ion per subunit.Cofactor: Binds 1 manganese ion per subunit.Subunit: PP1 comprises a catalytic subunit. PPP1CA. PPP1CB or PPP1CC. which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3. and then complexed to one or several targeting or regulatory subunits. PPP1R12A and PPP1R12B mediate binding to myosin. PPP1R3A. PPP1R3B. PPP1R3C and PPP1R3D mediate binding to glycogen. Interacts with cyanobacterial toxin microcystin; disulfide-linked. Interacts with PPP1R7.Subcellular Location: Cytoplasm.Miscellaneous: Microcystin toxin is bound to Cys-273 through a thioether bond.Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily. Chromosome Location: 12q24.1-q24.2 Sequence Position 1: 1 Sequence Position 2: 323 Sequence Length (aa): 323 MW: 36984 KEGG pathway: Focal adhesion 04510KEGG pathway: Insulin signaling pathway 04910KEGG pathway: Long-term potentiation 04720KEGG pathway: Regulation of actin cytoskeleton 04810Reactome Event:Lipid metabolism 73923 OMIM: 176914; gene. [ NCBI / EBI] Applications Tested: MS. SDS Applications Not Tested: ELISA. Western Blot Testing Protocols: SDS: Analysis of PPP1CC Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.||10-288-22549F_SD Cross-Reactivity: human Storage: -70°C. Avoid frequent freeze and thaw. Stability: 6-12 months at -70°C. Fusion: His-tag at N-terminus. Fusion: His-tag at N-terminus. Product ID: 5299