Ribonucleoside-diphosphate reductase M2 subunit

  • Ribonucleoside-diphosphate reductase M2 subunit

    CatalogueID : 10-288-22152F

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Target/Molecule Synonym Ribonucleotide reductase M2 polypeptide, EC 1.17.4.1, Ribonucleotide reductase small subunit, Ribonucleotide reductase small chain, RRM2, RR2, R2, RR2M
Format Purity: 0.95
Format: 10 mM Tris. pH 8.0. 0.1% Triton X-100. 0.002% NaN3. 10mM DTT.
Target Tag/Conjugate Unconjugated
Applications MS, SDS
References [1] Qiu. W. Zhou. B. Darwish. D. Shao. J. and Yen. Y. et al.
Characterization of enzymatic properties of human ribonucleotide reductase holoenzyme reconstituted in vitro from hRRM1. hRRM2. and p53R2 subunits
[2] Lin. Z. P. Belcourt. M. F. Cory. J. G. and Sartorelli. A. C. et al.
Stable suppression of the R2 subunit of ribonucleotide reductase by R2-targeted short interference RNA sensitizes p53(-/-) HCT-116 colon cancer cells to DNA-damaging agents and ribonucleotide reductase inhibitors
[3] Duxbury. M. S. Ito. H. Zinner. M. J. Ashley. S. W. and Whang. E. E. et al.
RNA interference targeting the M2 subunit of ribonucleotide reductase enhances pancreatic adenocarcinoma chemosensitivity to gemcitabine
[4] Shao. J. Zhou. B. Zhu. L. Qiu. W. Yuan. Y. C. Xi. B. and Yen. Y. et al.
In vitro characterization of enzymatic properties and inhibition of the p53R2 subunit of human ribonucleotide reductase
[5] Zhou. B. Liu. X. Mo. X. Xue. L. Darwish. D. Qiu. W. Shih. J. Hwu. E. B. Luh. F. and Yen. Y.
The human ribonucleotide reductase subunit hRRM2 complements p53R2 in response to UV-induced DNA repair in cells with mutant p53
[6] . Pavloff N. Rivard D. Masson S. Shen S. -H. Mes-Masson A. -M.
Sequence analysis of the large and small subunits of human ribonucleotide reductase.
[7] . Zhou B. Yen Y.
Characterization of the human ribonucleotide reductase M2 subunit gene; genomic structure and promoter analyses.
[8] . Hillier L. W. Graves T. A. Fulton R. S. Fulton L. A. Pepin K. H. Minx P. Wagner-McPherson C. Layman D. Wylie K. Sekhon M. et al.
Generation and annotation of the DNA sequences of human chromosomes 2 and 4.
[9] . Strausberg R. L. Feingold E. A. Grouse L. H. Derge J. G. Klausner R. D. Collins F. S. Wagner L. Shenmen C. M. Schuler G. D. Altschul S. F. et al.
Generation and initial analysis of more than 15. 000 full-length human and mouse cDNA sequences.
Accession Number Accession Number: NP_001025.1
Sp Accession Number: P31350
Source/Expression System E. coli
Original Item Name Ribonucleoside-diphosphate reductase M2 subunit
Activity Catalytic Activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Category Protein
Molecule Name Ribonucleoside-diphosphate reductase M2 subunit
EC Number EC 1.17.4.1
Unit 0.1 mg
Description Function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.Catalytic Activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.Cofactor: Binds 2 iron ions per subunit (By similarity).Pathway: DNA replication pathway; first step.Subunit: Heterodimer of a large (M1) and a small chain (M2).Subcellular Location: Cytoplasm.Disease: Ribonucleotide reductase is thought to mediate the pathogenesis of the immunodeficiency of adenosine deaminase or purine nucleoside phosphorylase. The deoxynucleotides that accumulate in the lymphoid cells of these patients are thought to feed-back inhibit ribonucleotide reductase. preventing DNA replication and cell proliferation.Miscellaneous: Two distinct regulatory sites have been defined: the specificity site. which controls substrate specificity. and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site. located on M1. is formed only in the presence of the second subunit M2.Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family.Summary: Ribonucleotide reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. It is composed of 2 non-identical subunits. proteins M1 and M2. Synthesis of M2 is regulated in a cell-cycle dependent fashion. Chromosome Location: 2p25-p24 Sequence Position 1: 1 Sequence Position 2: 389 Sequence Length (aa): 389 MW: 44878 KEGG pathway: Purine metabolism 00230KEGG pathway: Pyrimidine metabolism 00240Reactome Event:Cell Cycle (Mitotic) 69278Reactome Event:Nucleotide metabolism 15869 OMIM: 180390; gene. [ NCBI / EBI] Applications Tested: MS. SDS Applications Not Tested: ELISA. Western Blot Testing Protocols: SDS: Analysis of RRM2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.||10-288-22152F_SD Cross-Reactivity: human Storage: -70°C. Avoid frequent freeze and thaw. Stability: 6-12 months at -70°C. Fusion: T7 tag at N-terminus. Fusion: T7 tag at N-terminus. Product ID: 5119