Human Cleavage stimulation factor 50 kDa subunit

  • Human Cleavage stimulation factor 50 kDa subunit

    CatalogueID : GWB-8DBC1B

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Format Purity: 0.95
Format: 10 mM Tris. pH 8.0. 0.1% Triton X-100. 0.002% NaN3.
Target Tag/Conjugate Unconjugated
Target/Molecule Synonym Cleavage stimulation factor subunit 1, CSTF 50 kDa subunit, CF-1 50 kDa subunit, CstF-50, CstF-50, CstFp50
Species Human
Applications MS, SDS
Molecule Name Cleavage stimulation factor 50 kDa subunit
Source/Expression System E. coli
Accession Number NCBI Accession Number: NP_001315.1
Swiss Protein Accession Number: Q05048
Category Protein
Original Item Name Cleavage stimulation factor 50 kDa subunit
Unit 0.05 mg
Description Function: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.Subunit: Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit). CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). Interacts directly with CSTF3. Interacts with BARD1.Subcellular Location: Nucleus.Domain: The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3.Domain: N-terminus mediates homodimerization.Ptm: The N-terminus is blocked.Similarity: Contains 6 WD repeats.Summary: This protein is one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits. this protein contains transducin-like repeats. Several transcript variants with different 5' UTR. but encoding the same protein. have been found for this gene. Entry Name: CSTF1_HUMAN Chromosome Location: 20q13.31 Sequence Position 1: 1 Sequence Position 2: 431 Length (aa): 431 MW: 48358 Reactome Event:Gene Expression 74160Reactome Event:Transcription 74159Reactome Event:mRNA Processing 75071 OMIM: 600369; gene. [ NCBI / EBI] Applications: MS. SDS Applications Not Tested: ELISA, Western Blot Testing Protocols: SDS: Analysis of CSTF1 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.||10-288-22329F_SD Storage: -70°C. Avoid frequent freeze and thaw. Stability: 6-12 months at -70°C. Fusion: T7 tag at N-terminus.