Horse IgG F(c) fragment

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Original Item Name HORSE IgG F(c) fragment
Applications ELISA, Immunohistochemistry, Western Blot
Target Tag/Conjugate Unconjugated
Category Proteins and Peptides
Concentration 1.0 mg/mL
Keywords HORSE IgG F(c) fragment - 008-0103
Specifications/Features Horse IgG F(c) Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Horse IgG F(c) Fragment is stable at 4° C prior to restoration. It is recommended to aliquot restored Horse IgG F(c) Fragment and store at -20° C for extended storage and to prevent repeated freeze-thaw cycles
Purity Horse IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Horse IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c).  No reaction was observed against anti-Horse IgG F(ab’)2 or anti-Papain
Item Name Synonyms IgG F(c)
Format Lyophilized
Validated Applications WB, ELISA, IHC
Cite This Product Rockland Immunochemicals cat# 008-0103 RRID:AB_840586
Unit 1 mg
Description Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer posses the epitope recognition site