MethoCult™ M3231

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Applications Colony assays & quantification, Cell Culture
Material Methylcellulose-based
Method • Assays of mouse clonogenic hematopoietic progenitor cells in samples of bone marrow, spleen, peripheral blood and fetal liver, • Assays in which a specific combination of cytokines, growth factors, supplements or other compounds is desired
Accessories • Iscove's Modified Dulbecco's Medium (IMDM) with 2% Fetal Bovine Serum (Catalog #07700) • Blunt-end Needles (Catalog #28110/28120) • 3 cc Syringe (Catalog #28230/28240) • 35 mm Culture Dishes (Catalog #27100/27150) • 60 mm Gridded Scoring Dishes (Catalog #27500)
Species Mouse
Category Specialized cell culture media
Source/Expression System Hematopoietic stem & progenitor cells
Unit 80 mL
Description Methylcellulose medium without cytokines for mouse cellsMethoCult™ M3231 is an incomplete medium formulation that contains components selected for optimal growth of mouse hematopoietic colony-forming cells (CFCs). It is suitable for growth and detection of erythroid progenitors (BFU-E), granulocyte-macrophage progenitors (CFU-GM, CFU-M, CFU-G) and multi-potential granulocyte, erythroid, macrophage, megakaryocyte progenitors (CFU-GEMM) with the addition of appropriate growth factors and supplements. MethoCult™ M3231 can be used for the detection and quantification of hematopoietic progenitors in mouse bone marrow (BM), spleen, peripheral blood (PB) and fetal liver (FL) samples using CFC assays. This formulation does not contain erythropoietin or other cytokines