MethoCult™ GF M3434

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Applications Colony assays & quantification, Cell Culture
Material Methylcellulose-based
Method Colony-assays of mouse clonogenic hematopoietic progenitor cells in samples of bone marrow, spleen, peripheral blood and fetal liver
Accessories • Iscove's Modified Dulbecco's Medium (IMDM) with 2% Fetal Bovine Serum (Catalog #07700) • Blunt-end Needles (Catalog #28110/28120) • 3 cc Syringe (Catalog #28230/28240) • 35 mm Culture Dishes (Catalog #27100/27150) • 60 mm Gridded Scoring Dishes (Catalog #27500)
Species Mouse
Category Specialized cell culture media
Source/Expression System Hematopoietic stem & progenitor cells
Unit 24 x 3 mL
Description Pre-aliquoted methylcellulose medium with recombinant cytokines and EPO for mouse cellsMethoCult™ GF M3434 is optimized for the detection and quantification of mouse hematopoietic progenitors in bone marrow (BM), spleen, peripheral blood (PB) and fetal liver (FL) using CFC assays. MethoCult™ GF M3434 has been formulated to support optimal growth of erythroid progenitors (BFU-E), granulocyte-macrophage progenitors (CFU-GM, CFU-M, CFU-G) and multi-potential granulocyte, erythroid, macrophage, megakaryocyte progenitors (CFU-GEMM)