Human dermal melanocyte-light lysate

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Unit 200 µg
Description Cell lysate was prepared in modified RIPA buffer (50mM Tris, pH 7.2, 150mM NaCl, 1mM EDTA, 1mM EGTA, 1% NP-40, 1mM Na3VO4, 5mM NaF, 400uM PMSF, 1ug/ml of pepstatin A, 5ug/ml of aprotinin, 5ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was diluted with nonreducing sample buffer (50 mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue) containing 5% beta-mercaptoethanol.