Wizard SV Genomic DNA Purification Start-Up Kit, 230V Electrical (5...

  • Wizard SV Genomic DNA Purification Start-Up Kit, 230V Electrical (500 preps, manifold and free vacuum pump)

    from Promega
    CatalogueID : A6774

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Time 20 minutes
Specifications/Features

Starting Material:
cultured cells and tissue, including mouse tails

Starting Amount:
5 x 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip

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Yield 20–30µg of DNA per prep from 1.2cm mouse tail
Method membrane-based technique
Unit 1 each
Description The Wizard SV Genomic DNA Purification System provides a fast, simple, membrane-based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K (Cat.# V3021). Amplifiable genomic DNA can be isolated from up to 5 x 10(6) cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step. The Wizard SV Genomic DNA Purification System can be used in either a microcentrifuge (spin) or vacuum protocol. Up to 20 samples can be processed at once in the vacuum format with the Vac-Man Laboratory Vacuum Manifold (Cat.# A7231) and the Vacuum Adapters (Cat.# A1331).