• pFUSE-hIgG2e1-Fc1

    CatalogueID : pfc1-hg2e1

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Host can be transfected in a variety of mammalian cells, including myeloma cell lines, CHO cells, monkey COS cells and human embryonic kidney (HEK)293 cells, cells that are commonly used in protein purification systems.
Target Tag/Conjugate Fc region of human IgG2e1

Kit Contents:
20 µg of lyophilized DNA   •   4 pouches of E. coli Fast-Media©Zeo (2 TB and 2 Agar)

Vector Size:
4126 bp

Resistance Zeocin
Promoter EF1 prom/HTLV 5‘UTR driving the Fc fusion and CMV enh/FerL prom driving the selectable marker Zeocin
Vector Name pFUSE-hIgG2e1-Fc1
Origin of Replication minimal E. coli ori with same activity as the longer Ori.
Applications Expression and Purication on protein A or Protein G columns of fc fusion proteins
Unit 20 microg EA
Description pFUSE-hIgG2e1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG2 heavy chain mutated K322A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently. pFUSE-hIgG2e1-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.