pFUSE-hIgG3-Fc2

  • pFUSE-hIgG3-Fc2

    CatalogueID : pfuse-hg3fc2

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Host can be transfected in a variety of mammalian cells, including myeloma cell lines, CHO cells, monkey COS cells and human embryonic kidney (HEK)293 cells, cells that are commonly used in protein purification systems.
Target Tag/Conjugate Fc region of a human IgG3
Specifications/Features

Kit Contents:
20 µg of lyophilized DNA   •   4 pouches of E. coli Fast-Media©Zeo (2 TB and 2 Agar)

Applications Expression and Purication on protein A or Protein G columns of fc fusion proteins
Vector Name pFUSE-hIgG3-Fc2
Resistance Zeocin
Promoter EF1 prom/HTLV 5‘UTR driving the Fc fusion and CMV enh/FerL prom driving the selectable marker Zeocin
Origin of Replication minimal E. coli ori with same activity as the longer Ori.
Unit 20 µg
Description Plasmid for generating human Fc fusion proteins containing the IL-2 signal sequence. Fc-fusion proteins are chimeric proteins featuring the Fc region of an immunoglobulin fused to their C terminus. These soluble chimera retain the activity of the native protein and present the advantages of a long half-life in the circulatory system, efficient mammalian expression and ease of purification. Fc-fusion proteins are useful research tools for many applications and hold promise as therapeutics. pFUSE-Fc is a family of plasmids featuring Fc regions from various species: human IgG1, IgG2 and IgG4, mouse IgG2a, rabbit IgG, rat IgG2b, and engineered Fc from human IgG1, IgG2 and mouse IgG2a.