Human Macrophage Inflammatory Protein-3 beta (CCL19 MIP-3b)

  • Human Macrophage Inflammatory Protein-3 beta (CCL19 MIP-3b)

    CatalogueID : GWB-41DAAC

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Format Purity: Greater than 99% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Format: Recombinant macrophage inflammatory protein 3beta. lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
Protein Family: Belongs to the intercrine beta (chemokine CC) family.

Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC. using a standard
solution of MIP-3beta as a Reference Standard.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of MIP-3b.
Species Human
Target/Molecule Synonym Chemokine (C-C motif) ligand 19, CCL19, MIP-3b, ELC, MIP3B, SCYA19, Q6IBD6_HUMAN
Target Tag/Conjugate Unconjugated
Original Item Name Macrophage Inflammatory Protein-3 beta (CCL19. MIP-3b) Human
References [1] Weber.C. and Koenen.R.R. et al.
Fine-tuning leukocyte responses: towards a chemokine 'interactome'
[2] Hansson.M. Lundgren.A. Elgbratt.K. Quiding-Jarbrink.M. Svennerholm.A.M. and Johansson.E.L.
Dendritic cells express CCR7 and migrate in response to CCL19 (MIP-3beta) after exposure to Helicobacter pylori
[3] Giordano.D. Magaletti.D.M. and Clark.E.A. et al.
Nitric oxide and cGMP protein kinase (cGK) regulate dendritic-cell migration toward the lymph-node-directing chemokine CCL19
[4] Mishra.G.R. Suresh.M. Kumaran.K. Kannabiran.N. Suresh.S. Bala.P. Shivakumar.K. Anuradha.N. Reddy.R. Raghavan.T.M. et al.
Human protein reference database--2006 update
[5] Kaiser.A. Donnadieu.E. Abastado.J.P. Trautmann.A. and Nardin.A. et al.
CC chemokine ligand 19 secreted by mature dendritic cells increases naive T cell scanning behavior and their response to rare cognate antigen
[6] Ebert L. Schick M. Neubert P. Schatten R. Henze S. Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
[7] Clark G.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
Source/Expression System E. coli
Molecule Name Macrophage Inflammatory Protein-3 beta (CCL19 MIP-3b)
Category Protein
Unit 0.005 mg
Description Summary: This protein is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus. and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. Chromosome Location: 9p13 Sequence Length (aa): 98 MW: 10993KEGG pathway: Cytokine-cytokine receptor interaction 04060 OMIM: NCBI [ 602227]human Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Sequence: The sequence of the first five N-terminal amino acids was determined and wasfound to be Gly-Thr-Asn-Asp-Ala.Biological Activity: macrophage inflammatory protein 3beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human T cells using a concentration of 10-50 ng/ml.Solubility: It is recommended to reconstitute the lyophilized MIP-3b in sterile 18MolarOmega-cm H2O not less than 100ug/ml. which can then be further diluted to other aqueous solutions.Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Product ID: 45013