Mouse C-10 Protein

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Target Tag/Conjugate Unconjugated
Format Purity: Greater than 97% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Format: Murine C-10 was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives.
Species Mouse
Target/Molecule Synonym Small inducible cytokine A6, CCL6, C10 protein, C10, Scya6
Accession Number Swiss Protein Accession Number: P27784
Source/Expression System E. coli
References [1] Bai.J.. Lao.H. and Ye.X.. et al.
Molecular cloning and sequence analysis of insulin-like growth factor I cDNA from bluntsnout bream (Megalobrama amblycephala)
[2] Bai.J.. Lao.H.. Ye.X.. Li.Y. and Lou.J.. et al.
Direct Submission
[3] Bai J.. Lao H.. Ye X.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
Original Item Name C-10 Protein Murine
Category Protein
Molecule Name C-10 Protein
Unit 0.002 mg
Description Function: CCL6(22-95) and CCL6(23-95) are potent chemoattractants.Subcellular Location: Secreted protein.Tissue Specificity: Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF.Induction: Associated with stimuli that promote myeloid differentiation.Similarity: Belongs to the intercrine beta (chemokine CC) family. Entry Name: CCL6_MOUSE MW: 12984 Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Leu-Ile-Gln-Glu.Biological Activity: Murine C-10 is fully biologically active when compared to standard. The Biological activity is calculated by the chemotaxis assay using Balb/C mouse spleen MNCs. The maximum chemotactic activity was achieved at 100 ng/ml.Solubility: It is recommended to reconstitute the lyophilized Murine C-10 in sterile 18MolarOmega-cm H2O not less than 100ug/ml. which can then be further diluted to other aqueous solutions.Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.. Physical Appearance: Sterile Filtered White Lyophilized (freeze-dried) Powder. Protein Family: Belongs to the intercrine beta (chemokine CC) family..Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm .2. Analysis by RP-HPLC. using a calibrated solution of Murine C-10 as a Reference Standard.Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Murine C-10.