Mouse Interleukin-6 (IL-6)

  • Mouse Interleukin-6 (IL-6)

    CatalogueID : GWB-9854AF

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Species Mouse
Format Purity: Greater than 98% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Format: The protein was lyophilized from a concentrated (1 mg/ml) solution containg 50 mM NH4HCO3. pH8.0.
Protein Family: Belongs to the IL-6 superfamily.

Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC. using a standard solution of IL-6 as a Reference Standard.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of IL-6.
Target/Molecule Synonym IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL6, IL-6, IL6_HUMAN
Target Tag/Conjugate Unconjugated
Source/Expression System E. coli
Molecule Name Interleukin-6 (IL-6)
Original Item Name Interleukin-6 (IL-6) Mouse
References [1] Morgan.L. Cooper.J. Montgomery.H. Kitchen.N. and Humphries.S.E. et al.
The interleukin-6 gene -174G>C and -572G>C promoter polymorphisms are related to cerebral aneurysms
[2] Odenbreit.S. Linder.S. Gebert-Vogl.B. Rieder.G. Moran.A.P. and Haas.R.
Interleukin-6 induction by Helicobacter pylori in human macrophages is dependent on phagocytosis
[3] Meng.F. Yamagiwa.Y. Ueno.Y. and Patel.T. et al.
Over-expression of interleukin-6 enhances cell survival and transformed cell growth in human malignant cholangiocytes
[4] van den Berg.A. Freitas.J. Keles.F. Snoek.M. van Marle.J. Jansen.H.M. and Lutter.R.
Cytoskeletal architecture differentially controls post-transcriptional processing of IL-6 and IL-8 mRNA in airway epithelial-like cells
[5] Duan.H.O. and Simpson-Haidaris.P.J. et al.
Cell type-specific differential induction of the human gamma-fibrinogen promoter by interleukin-6
[6] Hirano T. Yasukawa K. Harada H. Taga T. Watanabe Y. Matsuda T. Kashiwamura S. Nakajima K. Koyama K. Iwamatsu A. et al.
Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin.
[7] Yasukawa K. Hirano T. Watanabe Y. Muratani K. Matsuda T. Nakai S. Kishimoto T.
Structure and expression of human B cell stimulatory factor-2 (BSF-2/IL-6) gene.
[8] May L.T. Helfgott D.C. Sehgal P.B.
Anti-beta-interferon antibodies inhibit the increased expression of HLA-B7 mRNA in tumor necrosis factor-treated human fibroblasts: structural studies of the beta 2 interferon involved.
[9] Zilberstein A. Ruggieri R. Korn J.H. Revel M.
Structure and expression of cDNA and genes for human interferon-beta-2. a distinct species inducible by growth-stimulatory cytokines.
[10] Brakenhoff J.P.J. de Groot E.R. Evers R.F. Pannekoek H. Aarden L.A.
Molecular cloning and expression of hybridoma growth factor in Escherichia coli.
Category Protein
Unit 0.002 mg
Description Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells.Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.Recombinant Murine IL-6 produced in E.Coli is a single. non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton. Mouse IL-6 is purified by proprietary chromatographic techniques.Function: IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells. it induces myeloma and plasmacytoma growth. it induces nerve cells differentiation. in hepatocytes it induces acute phase reactants.Subcellular Location: Secreted protein.Ptm: N- and O-glycosylated.Similarity: Belongs to the IL-6 superfamily. Chromosome Location: 7p21 Sequence Length (aa): 212 MW: 23718KEGG pathway: Cytokine-cytokine receptor interaction 04060KEGG pathway: Hematopoietic cell lineage 04640KEGG pathway: Jak-STAT signaling pathway 04630KEGG pathway: Toll-like receptor signaling pathway 04620 OMIM: 147620; gene. [ NCBI / EBI]mouse Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less then 0.02 ng/ml. corresponding to a Specific Activity of 1 x 10 7IU/mg.Solubility: It is recommended to reconstitute the lyophilized rmIL-6 in 100 mM acetic acid to 0.1-1.0 mg/mL to regain full activity. and can then be further diluted to other aqueous solutions.Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Product ID: 45181