Chicken Insulin Like Growth Factor II (cIGF-II)

  • Chicken Insulin Like Growth Factor II (cIGF-II)

    CatalogueID : GWB-CD19BD

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Format Purity: Greater than 95% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Format: The protein was lyophilized after extensive dialysis against 100 mM acetic acid.
Protein Family: Belongs to the insulin family.

Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC. using a standard solution of chicken IGF-II as a Reference Standard.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of rcIGF-II.
Target/Molecule Synonym IGF-II, Somatomedin A, IGF2, IGF-II, IGF2_HUMAN
Species Chicken
Target Tag/Conjugate Unconjugated
Molecule Name Insulin Like Growth Factor II (cIGF-II)
Category Protein
References [1] Fiedler.J. Brill.C. Blum.W.F. and Brenner.R.E. et al.
IGF-I and IGF-II stimulate directed cell migration of bone-marrow-derived human mesenchymal progenitor cells
[2] Watanabe.N. Nakadate.H. Haruta.M. Sugawara.W. Sasaki.F. Tsunematsu.Y. Kikuta.A. Fukuzawa.M. Okita.H. Hata.J. et al.
Association of 11q loss. trisomy 12. and possible 16q loss with loss of imprinting of insulin-like growth factor-II in Wilms tumor
[3] Monk.D. Sanches.R. Arnaud.P. Apostolidou.S. Hills.F.A. Abu-Amero.S. Murrell.A. Friess.H. Reik.W. Stanier.P. et al.
Imprinting of IGF2 P0 transcript and novel alternatively spliced INS-IGF2 isoforms show differences between mouse and human
[4] Murphy.S.K. Huang.Z. Wen.Y. Spillman.M.A. Whitaker.R.S. Simel.L.R. Nichols.T.D. Marks.J.R. and Berchuck.A.
Frequent IGF2/H19 domain epigenetic alterations and elevated IGF2 expression in epithelial ovarian cancer
[5] Maenaka.S. Hikichi.T. Imai.M.A. Minamoto.T. and Kawahara.E. et al.
Loss of imprinting in IGF2 in colorectal carcinoma assessed by microdissection
[6] Dull T.J. Gray A. Hayflick J.S. Ullrich A.
Insulin-like growth factor II precursor gene organization in relation to insulin gene family.
[7] Bell G.I. Merryweather J.P. Sanchez-Pescador R. Stempien M.M. Priestley L. Scott J. Rall L.B.
Sequence of a cDNA clone encoding human preproinsulin-like growth factor II.
[8] Shen S.-J. Daimon M. Wang C.-Y. Jansen M. Ilan J.
Isolation of an insulin-like growth factor II cDNA with a unique 5' untranslated region from human placenta.
[9] de Pagter-Holthuizen P. van Schaik F.M.A. Verduijn G.M. van Ommen G.J.B. Bouma B.N. Jansen M. Sussenbach J.S.
Organization of the human genes for insulin-like growth factors I and II.
[10] Irminger J.C. Rosen K.M. Humbel R.E. Villa-Komaroff L.
Tissue-specific expression of insulin-like growth factor II mRNAs with distinct 5' untranslated regions.
Source/Expression System E. coli
Original Item Name Insulin Like Growth Factor II (cIGF-II) Chicken
Unit 0.002 mg
Description Function: The insulin-like growth factors possess growth-promoting activity. In vitro. they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.Subcellular Location: Secreted protein.Mass Spectrometry: MW=7469.4; METHOD=MALDI; RANGE=25-91; NOTE=Ref.19. Ref.20.Mass Spectrometry: MW=7398.3; METHOD=MALDI; RANGE=26-91; NOTE=Ref.19. Ref.20.Similarity: Belongs to the insulin family.Summary: This protein is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through. INS-IGF2. which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Chromosome Location: 11p15.5 Sequence Length (aa): 180 MW: 20140 OMIM: 147470; gene+phenotype. [ NCBI / EBI]Chicken Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Biological Activity: rcIGF-II is fully biologically active when compared to standards. The ED50. was calculated 2 methods: 1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be less then 150 ng/ml. 2. Type 1 IGF receptor binding assay ED50 was found to be less then 20 ng/ml.Solubility: It is recommended to reconstitute the lyophilized rcIGF-II in sterile 18MolarOmega-cm H2O not less than 100ug/ml. which can then be further diluted to other aqueous solutions.Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Product ID: 45147