Human CD99

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Target/Molecule Synonym CD99 antigen (T-cell surface glycoprotein E2) (E2 antigen) (Protein MIC2) (12E7), CD99 antigen, T-cell surface glycoprotein E2, E2 antigen, Protein MIC2, 12E7, CD99, MIC2, MIC2X, CD99Y, MIC2Y
Species Human
Target Tag/Conjugate Unconjugated
Format Purity: Greater than 90% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.
Format: 100 ug/ml in 50 mM Tris-Acetate. pH7.5. 1 mM EDTA. 20% Glycerol without BSA and Sodium Azide.
Source/Expression System E. coli
Original Item Name CD99 Human
Accession Number NCBI Accession Number: P14209
Swiss Protein Accession Number: P14209
Category Protein
References [1] Shioda T.. Fenner M.H.. Isselbacher K.J.
msg1. a novel melanocyte-specific gene. encodes a nuclear protein and is associated with pigmentation.
[2] Strausberg R.L.. Feingold E.A.. Grouse L.H.. Derge J.G.. Klausner R.D.. Collins F.S.. Wagner L.. Shenmen C.M.. Schuler G.D.. Altschul S.F.. et al.
Generation and initial analysis of more than 15.000 full-length human and mouse cDNA sequences.
[3] Iwasaki T.. Chin W.W.. Ko L.
Identification and characterization of RRM-containing coactivator activator (CoAA) as TRBP-interacting protein. and its splice variant as a coactivator modulator (CoAM).
Molecule Name CD99
Unit 0.002 mg
Description CD99 is an integral membrane protein of 185 amino acids that is expressed highly on cells of the hematopoietic system and on Ewing tumor cells. CD99 has been implicated in cell adhesion and cell death and to participate through these processes in the differentiation of T-cell precursors at a critical stage of thymocyte differentiation when positive selection is known to occur (Bernard et al). Engagement of the CD99 molecule by CD99 monoclonal antibodies has been shown to induce homotypic aggregation of various cell types. CD99 is expressed constitutively on all peripheral blood T-cells and becomes further up-regulated upon cell activation . CD99 mediates TCR/CD3-dependent activation of resting peripheral blood T-cells and specifically induces the production of the Th1 cytokines TNF-alpha and IFN-gamma.Recombinant CD99 shows a band at 18kDa on SDS-PAGE commassie blue stained gel. Recombinant CD99 is purified by proprietary chromatographic techniques.Function: Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.Subcellular Location: Membrane; single-pass type I membrane protein (Potential).Ptm: Extensively O-glycosylated.Miscellaneous: The gene encoding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes.Similarity: Belongs to the CD99 family. Entry Name: CD99_HUMAN Length (aa): 185 MW: 18848 OMIM: 313470; gene. [ NCBI / EBI]450000; gene. [ NCBI / EBI] Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Aggregates: Less than 1% as determined by coomassie blue SDS-PAGE gel analysis. Physical Appearance: Sterile filtered Liquid. Protein Family: Belongs to the CD99 family.Endotoxin: Less than 0.1 ng/ug (IEU/µg) of CD99.