Human Artemin (ART)

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Species Human
Format Purity: Greater than 98% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Format: Recombinant Artemin was lyophilized after extensive dialysis against 10 mM sodium citrate pH-4.5 and 25 mM sodium chloride.
Target Tag/Conjugate Unconjugated
Target/Molecule Synonym Neurotrophic factor artemin isoform 1., Artemin, Enovin, Neublastin, Artemin
Accession Number NCBI Accession Number: NP_003967.1
Swiss Protein Accession Number: Q5T4W7
Original Item Name Artemin (ART) Human
Category Protein
Source/Expression System E. coli
Molecule Name Artemin (ART)
Unit 0.005 mg
Description Function: Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.Subunit: Homodimer; disulfide-linked (By similarity).Subcellular Location: Secreted protein (By similarity).Tissue Specificity: Ubiquitous. Expressed at high levels in peripheral tissues including prostate. placenta. pancreas. heart. kidney. pituitary gland. lung and testis. Expressed at low levels in the brain.Developmental Stage: Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain.Similarity: Belongs to the TGF-beta family. GDNF subfamily.Summary: The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenrative disease. Artemin has been shown in culture to support the survival of a number of periferal neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Four alternatively spliced transcripts have been described. two of which encode the same protein. Entry Name: ARTN_HUMAN Chromosome Location: 1p33-p32 Length (aa): 220 MW: 22878 OMIM: 603886; gene. [ NCBI / EBI] Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Gly-Gly-Pro-Gly.Biological Activity: Artemin is fully biologically active when compared to standard. The ED50. calculated by the dose-dependant proliferation assay SH-SY5Y cell line which was found to be 4-8ng/ml. The activity can also determined by it`s ability to promote survival and neurite outgrowth.Solubility: It is recommended to reconstitute the lyophilized Human Artemin in sterile 18MolarOmega-cm H2O not less than 100ug/ml. which can then be further diluted to other aqueous solutions.Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Physical Appearance: Sterile Filtered White Lyophilized (freeze-dried) Powder. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC. using a calibrated solution of Artemin as a Reference Standard.Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Human Artemin.