Human Casein Kinase 2 beta (CK2-beta)

  • Human Casein Kinase 2 beta (CK2-beta)

    CatalogueID : GWB-4B6793

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Format Purity: Greater than 95% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Format: Recombinant Human CK2b is lyophilized from 25 mM Tris-HCl. 500 mM NaCl. 1 mM DTT. 1 mM EDTA. 500 uM AEBSF. 1 uM leupeptin. 1 ug/ml aprotinin. pH8.5. Solubilize in distilled H2O or 50%glycerol.
Species Human
Target/Molecule Synonym Casein kinase 2. beta polypeptide, Casein kinase II subunit beta, CK II beta, Phosvitin, G5a, CK2b, CK2N G5A
Target Tag/Conjugate Unconjugated
Source/Expression System E. coli
Category Protein
References [1] Wilkinson.J.M.. et al.
Troponin C from rabbit slow skeletal and cardiac muscle is the product of a single gene
[2] Putney.S.D.. Herlihy.W.C. and Schimmel.P.. et al.
A new troponin T and cDNA clones for 13 different muscle proteins. found by shotgun sequencing
[3] Wilkinson J.M.
Troponin C from rabbit slow skeletal and cardiac muscle is the product of a single gene.
[4] Putney S.D.. Herlihy W.C.. Schimmel P.R.
A new troponin T and cDNA clones for 13 different muscle proteins. found by shotgun sequencing.
Molecule Name Casein Kinase 2 beta (CK2-beta)
Original Item Name Casein Kinase 2 beta (CK2-beta) Human
Accession Number NCBI Accession Number: NP_001311.3
Swiss Protein Accession Number: P67870
Unit 0.003 mg
Description Function: Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.Subunit: Tetramer composed of an alpha subunit. an alpha' subunit and two beta subunits. Interacts with CD163. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1. SUPT16H. CSNK2A1. CSNK2A2 and CSNK2B. the complex associating following UV irradiation.Ptm: Phosphorylated by alpha subunit.Similarity: Belongs to the casein kinase 2 subunit beta family.Summary: This protein is the beta subunit of casein kinase II. a ubiquitous protein kinase which regulates metabolic pathways. signal transduction. transcription. translation. and replication. The enzyme is composed of three subunits. alpha. alpha prime and beta. which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic. while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Entry Name: CSK2B_HUMAN Length (aa): 215 MW: 24942 KEGG pathway: Adherens junction 04520KEGG pathway: Tight junction 04530KEGG pathway: Wnt signaling pathway 04310 OMIM: 115441; gene. [ NCBI / EBI] Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Biological Activity: specific activity > 1U/mg.Solubility: It is recommended to reconstitute the lyophilized rHuCK2b in sterile 18MolarOmega-cm H2O not less than 100ug/ml. which can then be further diluted to other aqueous solutions.Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Physical Appearance: Sterile Filtered White Lyophilized (freeze-dried) Powder. Protein Family: Belongs to the casein kinase 2 beta subunit family.