Protein kinase C zeta type

  • Protein kinase C zeta type

    CatalogueID : GWB-D8CAAB

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Target/Molecule Synonym Protein kinase C. zeta isoform 2, EC 2.7.11.13, nPKC-zeta, PRKCZ, PKC2
Target Tag/Conjugate Unconjugated
EC Number EC 2.7.11.13
References [1] Zhang. Y. Liao. M. and Dufau. M. L. et al.
Phosphatidylinositol 3-kinase/protein kinase Czeta-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene
[2] Even-Faitelson. L. and Ravid. S. et al.
PAK1 and aPKCzeta regulate myosin II-B phosphorylation: a novel signaling pathway regulating filament assembly
[3] Sajan. M. P. Rivas. J. Li. P. Standaert. M. L. and Farese. R. V. et al.
Repletion of atypical protein kinase C following RNA interference-mediated depletion restores insulin-stimulated glucose transport
[4] Xie. Z. Dong. Y. Zhang. M. Cui. M. Z. Cohen. R. A. Riek. U. Neumann. D. Schlattner. U. and Zou. M. H.
Activation of protein kinase C zeta by peroxynitrite regulates LKB1-dependent AMP-activated protein kinase in cultured endothelial cells
[5] Brady. S. C. Allan. L. A. and Clarke. P. R. et al.
Regulation of caspase 9 through phosphorylation by protein kinase C zeta in response to hyperosmotic stress
[6] Kochs G. Hummel R. Meyer D. Hug H. Marme D. Sarre T. F.
Activation and substrate specificity of the human protein kinase C alpha and zeta isoenzymes.
[7] Barbee J. L. Loomis C. R. Deutscher S. L. Burns D. J.
The cDNA sequence encoding human protein kinase C-zeta.
[8] Kalnine N. Chen X. Rolfs A. Halleck A. Hines L. Eisenstein S. Koundinya M. Raphael J. Moreira D. Kelley T. et al.
Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
[9] Gregory S. G. Barlow K. F. McLay K. E. Kaul R. Swarbreck D. Dunham A. Scott C. E. Howe K. L. Woodfine K. Spencer C. C. A. et al.
The DNA sequence and biological annotation of human chromosome 1.
[10] Sanchez P. De Carcer G. Sandoval I. V. Moscat J. Diaz-Meco M. T.
Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62.
Activity Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Original Item Name Protein kinase C zeta type
Molecule Name Protein kinase C zeta type
Accession Number Accession Number: NP_001028753.1
Sp Accession Number: Q05513
Category Protein
Source/Expression System Insect Cells
Unit 0.001 mg
Description Recombinant full-length human PKC zeta was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.Function: This is a calcium-independent. phospholipid-dependent. serine- and threonine-specific enzyme.Function: PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters. a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.Enzyme Regulation: Phosphatidylinositol 3.4.5-trisphosphate might be a physiological activator.Subunit: Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 (By similarity). Interacts with PARD6A. PARD6B. PARD6G and SQSTM1. Part of a complex with PARD3. PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1 (Probable). Interacts with IKBKB.Subcellular Location: Cytoplasm. In the retina. localizes in the terminals of the rod bipolar cells (By similarity). Endosome. Associates with endosomes.Tissue Specificity: Expressed in brain. and to a lesser extent in lung. kidney and testis.Domain: The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.Similarity: Belongs to the Ser/Thr protein kinase family. PKC subfamily.Similarity: Contains 1 OPR domain.Similarity: Contains 1 phorbol-ester/DAG-type zinc finger.Similarity: Contains 1 protein kinase domain.Summary: Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation. differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent. PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore. it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes. it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to. but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. Sequence Length (aa): 409 MW: 67660 KEGG pathway: Insulin signaling pathway 04910KEGG pathway: Tight junction 04530KEGG pathway: Type II diabetes mellitus 04930 OMIM: 176982; gene. [ NCBI / EBI] Testing Protocols: Specific Activity: ||10-782-55107_1!!Purity: ||10-782-55107_2 Storage: Store product at -70°C. For optimal storage. aliquot target into smaller quantities after centrifugation and store at recommended temperature. Stability: 1 year at -20°C. Product ID: 55107