StemSpan™ SFEM

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Applications Expansion, Cell Culture
Material Liquid, Serum-free
Method • Culture and expansion of human hematopoietic progenitors in samples of bone marrow, cord blood and mobilized peripheral blood, • Expansion of mouse colony-forming cells, long-term culture-initiating cell (LTC-IC) and long-term repopulating cells, • Generation and culture of human and mouse dendritic cells, • Culture of rat and non-human primate cells
Species Human, Mouse
Category Specialized cell culture media
Source/Expression System Dendritic cells, Hematopoietic stem & progenitor cells, Monocytes
Unit 500 mL
Description Serum-free medium for expansion of hematopoietic cellsStemSpan™ Serum-Free Expansion Medium (SFEM) has been developed for the in vitro culture and expansion of human hematopoietic cells. This medium contains pre-tested bovine serum albumin, insulin and transferrin, and supplements in Iscove’s MDM. Recombinant hematopoietic growth factors, required for the optimal growth and expansion of hematopoietic cells, have not been added to StemSpan™ SFEM. This allows users the flexibility to prepare medium that meets their requirements. In addition to the culture and expansion of human hematopoietic cells, StemSpan™ SFEM has been used for the culture and expansion of hematopoietic stem and progenitor cells from other species, including mouse, non-human primate and dog. StemSpan™ SFEM has also been used for the generation, culture and assay of human and mouse dendritic cells, and for the culture of various other hematopoietic and non-hematopoietic cell types. In most applications, addition of specific hematopoietic growth factors, cytokines and/or other compounds is required for optimal growth