The Anti-HisG Antibody is a purified mouse monoclonal antibody designed to specifically detect recombinant proteins containing the seven amino acid 6xHis-Gly epitope. This epitope is present in many of Invitrogen's expression vectors encoding an N-terminal 6xHis tag. The Anti- HisG Antibody recognizes the following sequence:
-His-His-His-His-His-His-Gly-
The Anti-HisG Antibody has been functionally tested for use in western blots, ELISA, and immunoprecipitation.
Recommended dilution for western blot: 1:5,000
Antibody subclass: IgG2a