Human GPNMB protein

  • Human GPNMB protein

    from biorbyt
    CatalogueID : orb49688

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Applications SDS-PAGE
Format Lyophilized from a 0.2 µM filtered solution of 20mM PB, 150mM NaCl, pH 7.2
Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE
Species Human
Host HEK293
Category Proteins
Target/Molecule Synonym Transmembrane Glycoprotein NMB protein, Transmembrane Glycoprotein HGFIN protein, GPNMB protein, HGFIN protein, NMB protein
Specifications/Features Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.



Application Dilution: HEK293

Biological Origin: HEK293



Application Notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Validated Applications Western Blot (WB)
Validated Species Reactivity Human (Hu)
Molecule Name GPNMB
Sequence AKRFHDVLGN ERPSAYMREH NQLNGWSSDE NDWNEKLYPV WKRGDMRWKN SWKGGRVQAV LTSDSPALV GSNITFAVNL IFPRCQKEDA NGNIVYEKNC RNEAGLSADP YVYNWTAWSE D SDGENGTG QSHHNVFPDG KPFPHHPGWR RWNFIYVFHT LGQYFQKLGR CSVRVSVNTA NV TLGPQLM EVTVYRRHGR AYVPIAQVKD VYVVTDQIPV FVTMFQKNDR NSSDETFLKD LPI MFDVLI HDPSHFLNYS
Unit 10 ug, 50 ug, 1 mg
Description Recombinant Human Transmembrane Glycoprotein NMB/GPNMB produced by transfected human cell is a secreted protein with sequence (Ala22Pro486) of Human GPNMB fused with a polyhistidine tag at the Cterminus.Osteoactivin is an intracellular glycoprotein belongs to the NMB/pMEL17 family, which is asscociated with cell endosomal/lysomal compartments. Human Osteoactivin is a 560 amino acid type I transmembrane protein, and one alternate splice form shows a 12 amino acid insert between amino acid 339340. An additional 206 amino acid isoform shows a mutation at position 181 that results in a 26 amino acid substitution for the Cterminal 380 amino acids. Cells knowns to express Osteoactivin include fibroblast, osteoblasts, myeloid dendritic cell, melanocytes, plus fetal chondrocytes and stratum basale keratinocytes, macrophages/keratinocytes