Neprilysin (human), (recombinant) (His-tag)

  • Neprilysin (human), (recombinant) (His-tag)

    CatalogueID : BML-SE532-0010

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Format Liquid. In 50mM TRIS, pH 8.0. containing 300mM sodium chloride and 20% glycerol
Molecular Weight 80.7 kDa (calculated) / ~85 kDa (glycosylated form on SDS-PAGE)
Purity ≥95% (SDS-PAGE)
EC Number 3.4.24.11
Item Name Synonyms NEP| Neutral endopeptidase 24.11| Enkephalinase| CALLA| Common acute lymphoblastic leukemia antigen| CD10
Activity 1140 pmol/min/µg (9ng NEP were used to cleave 10µM Mca-RPPGFSAFK(Dnp)-OH (Fluorogenic Substrate) (Prod. No. BML-P227) at 37°C in 50mM TRIS, pH 9.0)
Brand/Trademark BIOMOL
Applications Study enzyme kinetics, cleave target substrates, and screen for inhibitors
Specifications/Features

Mainfeature: Highly active

Target/Molecule Synonym Neprilysin
Sequence NEP from human cDNA, transcript variant 1, aa 53-750 (D53 - W750; Identical to GeneBank accession NM_000902) is fused at the N-terminus to a His-tag
Keywords Recombinant protein| Enzyme IMM/CDM| NEU/NED| PRT/NLY| SIG/PRO/NLY| PSS/DEG/PRD Neprilysin| Neprilysin
Molecule Name Neprilysin
Source/Expression System Produced in Sf9 cells
Original Item Name Neprilysin (human), (recombinant) (His-tag)
Category Recombinant protein: Enzyme
Entrez GeneID 4311
Uniprot ID P08473
NCBI Full Gene Name membrane metallo-endopeptidase
NCBI Gene Aliases CALLA, CD10, NEP, SFE
Unit 10 µg
Description Neprilysin is a glycosylated zinc endopeptidase whose physiological targets include bradykinin, glucagon, GLP-1, and neuropeptides. Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1