Human Insulin Like Growth Factor Binding Protein-1 (IGFBP-1)

  • Human Insulin Like Growth Factor Binding Protein-1 (IGFBP-1)

    CatalogueID : GWB-8E4CA2

  • Contact Vendor

Target Tag/Conjugate Unconjugated
Species Human
Format Purity: Greater than 95% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Format: IGFBP-1 was lyophilized with 10 mM HCL.
Protein Content: Protein quantitation was carried out by two independent methods:
1.UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC. using a standard
solution of IGFBP-1 as a Reference Standard.

Endotoxin: Less than 0.1 ng/ug (IEU/ug) of IGFBP-1 .
Target/Molecule Synonym IGFBP-1, IBP-1, IGF-binding protein 1, Placental protein 12, PP12, IGFBP1, IGFBP-1, IBP1, IBP1_HUMAN
Category Protein
Molecule Name Insulin Like Growth Factor Binding Protein-1 (IGFBP-1)
Source/Expression System NSO Cells
Original Item Name Insulin Like Growth Factor Binding Protein-1 (IGFBP-1) Human
References [1] Brinkman A. Groffen C. Kortleve D.J. Geurts A. Drop S.L.S.
Isolation and characterization of a cDNA encoding the low molecular weight insulin-like growth factor binding protein (IBP-1).
[2] Brewer M.T. Stetler G.L. Squires C.H. Thompson R.C. Busby W.H. Jr. Clemmons D.R.
Cloning. characterization. and expression of a human insulin-like growth factor binding protein.
[3] Grundmann U. Nerlich C. Bohn H. Rein T.
Cloning of cDNA encoding human placental protein 12 (PP12): binding protein for IGF I and somatomedin.
[4] Julkunen M. Koistinen R. Aalto-Setala K. Seppala M. Janne O.A. Kontula K.
Primary structure of human insulin-like growth factor-binding protein/placental protein 12 and tissue-specific expression of its mRNA.
[5] Lee Y.-L. Hintz R.L. James P.M. Lee P.D.K. Shively J.E. Powell D.R.
Insulin-like growth factor (IGF) binding protein complementary deoxyribonucleic acid from human HEP G2 hepatoma cells: predicted protein sequence suggests an IGF binding domain different from those of the IGF-I and IGF-II receptors.
[6] Cubbage M.L. Suwanichkul A. Powell D.R.
Structure of the human chromosomal gene for the 25 kilodalton insulin-like growth factor binding protein.
[7] Brinkman A. Groffen C.A. Kortleve D.J. Drop S.L.
Organization of the gene encoding the insulin-like growth factor binding protein IBP-1.
[8] Ehrenborg E. Larsson C. Stern I. Janson M. Powell D.R. Luthman H.
Contiguous localization of the genes encoding human insulin-like growth factor binding proteins 1 (IGBP1) and 3 (IGBP3) on chromosome 7.
[9] Rieder M.J. Livingston R.J. Daniels M.R. Chung M.-W. Miyamoto K.E. Nguyen C.P. Nguyen D.A. Poel C.L. Robertson P.D. Schackwitz W.S. et al.
NIEHS-SNPs. environmental genome project. NIEHS ES15478. Department of Genome Sciences. Seattle. WA (URL: http://egp.gs.washington.edu).
[10] Kalnine N. Chen X. Rolfs A. Halleck A. Hines L. Eisenstein S. Koundinya M. Raphael J. Moreira D. Kelley T. et al.
Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
Unit 0.005 mg
Description IGFBP-1 is one of the six homologous proteins that specifically bind insulin-like growth factors and modulate their mitogenic and metabolic actions. The function of this protein is not completely defined. However. several studies demonstrate that it inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actionsInsulin Like Growth Factor Binding Protein modulate IGF activities by increasing their plasma half lives and by inhibiting or promoting the interactions of IGF with receptors on certain target cells. In addition these binding proteins provide a reservoir for IGF in pericellular spaces. Some IGF-BPs also have stimulating effects in vitro and some may inhibit the growth of cells.Recombinant Human Insulin Like Growth Factor Binding Protein-1 is a single glycosylated polypeptide chain containing 218 amino acids and having a molecular mass of 28806 Dalton. The IGFBP-1 is purified by proprietary chromatographic techniques.Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.Subunit: Binds equally well IGF1 and IGF2.Subcellular Location: Secreted protein.Ptm: Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.Similarity: Contains 1 IGFBP domain.Similarity: Contains 1 thyroglobulin type-1 domain. Sequence Length (aa): 259 MW: 27904 OMIM: 146730; gene. [ NCBI / EBI]Human Storage: 4°C for short term (weeks) and -20°C for long term. Avoid frequent freeze and thaw. Stability: 5 years at -20°C. Biological Activity: IGFBP-1 is fully biologically active when compared to standards. Inhibition of IGF-I stimulated proliferation of NUC-1 cells:IC50 ~ 100 ng/ml Inhibition of serum-induced stimulation of DNA synthesis in chick embryo fibroblast primary cultures:IC50 (1% serum) ~300 ng/ml.Solubility: It is recommended to reconstitute the lyophilized IGFBP-1 in sterile 18MolarOmega-cm H2O not less than 100ug/ml. which can then be further diluted to other aqueous solutions.Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Product ID: 45148