Brain Slice Keeper and Holder

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Unit Pair
Description This item is the HOLDER (beaker #BSKH) and Brain Slice Keeper (S-BSK). Slices maintained for many hours prior to transfer to recording chamber Simple to set up and maintain Slices supported on a quick-change nylon net The Brain Slice Keeper has been designed to pre-incubate brain slices prior to transfer into recording chambers. It consists of a pair of closely fitting acrylic rings, upper and lower, between which is wedged a sheet of nylon netting. In operation, it is totally immersed in an ACSF-filled holding vessel (BSKH) to which a 95% O2, 5% CO2 gas mixture is being supplied. The bubbles rising from the base of the BSKH saturate the ACSF and provide constant circulation of medium to the slices which rest on a nylon net. Since the lower ring carries a deflector shield, bubbles are prevented from being trapped under the slices and ensure continuous circulation of medium. Slices remain viable for many hours in these conditions. The typical fluid volume with BSK1 with a BSKH is 220ml. Now includes a needle valve for adjusting gas flow.