OneTaq Quick-Load 2X Master Mix with Standard Buffer

  • OneTaq Quick-Load 2X Master Mix with Standard Buffer

    CatalogueID : M0486S

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Specifications/Features

Information:
Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.

Concentration 2 X
Unit 100 rxns
Description OneTaq Quick-Load 2X Master Mix with Standard Buffer - 100 rxns OneTaq™ Quick-Load® 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep VentR ™ DNA Polymerases ideally suited to routine PCR applications from templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase . The convenient quick-load master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.