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Regulatory Status For Research Use Only. Not for any animal or human therapeutic or diagnostic use.
Applications Cell Analysis:Cell Metabolism:Endocytosis, Exocytosis & Phagocytosis:Nitro-Oxidative Stress:Reactive Oxygen Species:Cell Viability, Proliferation & Function
Keywords Free, Radical, _KWCPNT, MT1534, MT38433, MT38842, KWCPNT_
Unit 5 mg
Description OxyBURST; Green H2DCFDA succinimidyl ester (2',7'-dichlorodihydrofluorescein diacetate, SE) is an amine-reactive assay reagent that can be used to prepare oxidation-sensitive conjugates of a wide variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextrans, bacteria, yeast and polystyrene microspheres. Following conjugation to amines, the two acetates of OxyBURST; Green H2DCFDA can be removed by treatment with hydroxylamine at neutral pH to yield the dihydrofluorescein conjugate. OxyBURST; Green H2DCFDA conjugates are nonfluorescent until they are oxidized to the corresponding fluorescein derivatives.ROS Indicator Specifications:; Ex/Em: ~492495/517527 nm; Product is air sensitive and should be stored under dry argon or nitrogen; Product may be dissolved in DMSO, DMF, or ethanol for use; Indicator is cell permeant (cell loading protocols are available in the literature); Fluorescence can be monitored using a flow cytometer, fluorometer, micro